Immunocytochemical Localization of Basic Fibroblast Growth Factor in the Rat Pituitary Gland.
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منابع مشابه
Immunocytochemical localization of basic fibroblast growth factor in the human pituitary gland.
The immunocytochemical localization of basic fibroblast growth factor (bFGF) was studied in the human pituitary gland using a polyclonal antibody against fraction 1-24 of bovine recombinant bFGF. From a technical perspective, methacarn-fixed tissues were associated with a better preservation of bFGF immunoreactivity. Basic FGF-immunopositive glandular secretory cells were detected from the feta...
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Basic fibroblast growth factor has 4 cysteine residues in its amino acid sequence, two of which are perfectly conserved within the fibroblast growth factor family of proteins suggesting a disulfide bond at this position. Furthermore, thiol titration of bovine pituitary basic fibroblast growth factor (bFGF) indicates the presence of two free thiols, which is consistent with an intramolecular dis...
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The presence and distribution of basic fibroblast growth factor (bFGF) and transforming growth factor beta 1 in benign and malignant human breast tissue were determined by immunohistochemistry and immunoblotting. Peroxidase staining of biopsy specimens using a polyclonal antibody to amino acids 1-24 of bFGF and a monoclonal antibody to whole recombinant bFGF showed this growth factor to be loca...
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The immunohistochemical localization of basic fibroblast growth factor (FGF-2) in the submandibular glands of the rat was investigated by use of an antiserum to FGF-2. Nerve fiber bundles with FGF-2-immunoreactivity were found in association with interlobular ducts and blood vessels; they dissociated into single immunoreactive nerve fibers perhaps to terminate in proximity to acinar cells, or t...
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Cultured monolayers of bovine pituitary follicular cells, which transport ions, contain high amounts of mitogenic activity for endothelial cells which, on the basis of gene expression analysis, heparin-Sepharose elution profile, bioassay, immunoblotting, radioimmunoassay, and radioreceptor assay, has been identified as basic fibroblast growth factor (bFGF). These data indicate that follicular c...
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ژورنال
عنوان ژورنال: Archives of Histology and Cytology
سال: 1993
ISSN: 0914-9465,1349-1717
DOI: 10.1679/aohc.56.269